Fig. 5

MiR-152-3p exerts it’s radioresistant effect on CC cells by targeting KLF15. A qRT-PCR analysis verified the expression of KLF15 knockdown by transfecting shRNA in SiHa and Hela cells. **P < 0.01 versus NC group. B The apoptosis in KLF15 knockdown CC cells was significantly reduced compared with control after 72 h of radiotherapy by flow cytometry. **P < 0.01 versus NC group. C The expression of γ-H2AX after transfection in KLF15 knockdown CC cells was significantly reduced compared with control by qRT-PCR. *P < 0.05 and **P < 0.01 versus NC group. D qRT-PCR verified the expression of KLF15 in SiHa and Hela cells after different transfections as indicated and significant difference was found between miR-152-3p overexpression group and NC group. **P < 0.01 and ***P < 0.001 versus NC group. E The proliferation of SiHa and Hela cells after different transfections was shown by CCK-8 assay and significant difference was found between miR-152-3p overexpression group and NC group. **P < 0.01 versus NC group. F Cell migration in SiHa and Hela cells was measured by transwell assay after different transfections. Representative images are shown for NC, miR152-3p and miR152-3p + KLF15 from 3 independent experiments (n = 3), scale bar: 20 μm. G The apoptosis assay of SiHa and Hela cells after different transfections was shown by flow cytometry, and the significant difference was found between miR-152-3p overexpression group and NC group. **P < 0.01 and ***P < 0.001 versus NC group. H The expression of γ-H2AX in SiHa and Hela cells after different transfections was quantified by qRT-PCR and the significant difference was found between miR-152-3p overexpression group and NC group. *P < 0.05 versus NC group. *P < 0.05 and **P < 0.01 versus NC group