Fig. 3

MiR-152-3p acts as an effector cargo molecule in hypoxic cells-derives sEVs in CC radiation response. A The expression levels of miR-152-3p in SiHa and Hela CC cells after HE-sEVs or NE-sEVs treatment was examined by qRT-PCR. ***P < 0.001 versus negative control CC or NE-sEV cells. B The different expressions of sEV miR-152-3p in SiHa and Hela CC cells treated with NC + RNase R (0.1 mg/mL), HE-sEVs + RNase R or NE-sEVs + RNase R were shown by qRT-PCR. ***P < 0.001 versus CC cells sEVs treated with RNase R. C qRT-PCR was used to detect the expression of miR-152-3p in sEVs treated with different oxygen concentrations and destroyed with TritonX-100 (0.3%) and RNase R (0.1 mg/ml). D The level of miR-152-3p in SiHa and Hela cells is correlated with the time and dose substitution of HE-sEVs treatment. E Inhibition of miR-152-3p by sh-miR-152-3p transfection in HE-sEVs was confirmed by qRT-PCR. ***P < 0.001 versus NC group, ^###P < 0.001 versus HE-sEVs group. F Effects of different treatments on proliferation of SiHa and HeLa cells was measured by CCK-8 assay. **P < 0.01, ***P < 0.001 versus NC group, ^##P < 0.01, ^###P < 0.001 versus H-EV group. G Transwell assay of SiHa and Hela cells with different treatments was conducted, and the representative images were shown. *P < 0.05, **P < 0.01 versus NC group, ^#P < 0.05, ^##P < 0.01 vs HE-sEVs group. H The effects of different treatments on apoptosis of SiHa and HeLa cells were analyzed by flow cytometry. ***P < 0.001 versus NC group, ^###P < 0.001 versus HE-EVs group. I The level of γ-H2AX expression in SiHa and Hela cells with different treatments was quantified. All irradiation treatment doses used were 4 Gy. *P < 0.05, **P < 0.01 versus NC group, ^#P < 0.05 versus HE-sEVs group