Fig. 2

The role of miR-152-3p in CC radiation response. A The differentially expressed miRNAs from GSE81137 database were shown by volcanic map. B The level of miR-152-3p expression in cervical tumor tissues compared to adjacent tissues was examined by qRT-PCR, n = 20 in each group. *P < 0.05 versus adjacent tissues. C The significant difference of sEVs miR-152-3p expression was found in SiHa and Hela cells with different oxygen contents (normoxia and hypoxia) by qRT-PCR. **P < 0.01 versus CC cells with normoxia. D A significant difference of miR-152-3p expression was found after miR-152-3p mimic treatment on SiHa and Hela CC cells by qRT-PCR, **P < 0.01 versus negative control CC cells. E Flow cytometry was conducted to detect the effect of miR-152-3p mimics on radiation-induced apoptosis in SiHa and Hela cells. **P < 0.01 versus negative control CC cells. F The significant difference of expression of the DNA damage markers γ-H2AX was shown by immunofluorometric assay after miR-152-3p mimic treatment on SiHa and Hela CC cells. **P < 0.01 and ***P < 0.001 versus negative control CC cells. G The reduced expression of γ-H2AX and p-DNApkcs was shown by western blot in SiHa and Hela cells treated with miR-152-3p mimic or negative control (NC). H Hela cells transfected with miR-152-3p mimics and negative control (NC) plasmid were injected into nude mice, respectively, tumor volume and weight after irradiation were measured, n = 6 in each group. **P < 0.01 versus negative control CC cells. I Representative images of HE, Ki67 and TUNEL staining with NC + IR and miR-152-3p + IR, scale bar: 20 μm. All irradiation treatment doses used were 4 Gy. Right panel shows the quantification of Ki67 and TUNEL positive cells in two treatment groups. *P < 0.05 and **P < 0.01 versus negative control CC cells