Fig. 9

Nuclear γH2AX foci analysis of A549. A549 cells were fixated 0.5 h and 24 h subsequent to irradiation with 2 Gy A photons or proton irradiation administered at a B proximal and a C distal position of a SOBP. The baseline level of γH2AX foci (3.7 foci/cell) in unirradiated A549 cells is illustrated by a dotted threshold line as reference. Ganetespib or sham treatment (0 nM) was administered 1 h prior to irradiation and replaced by fresh drug-containing medium after the irradiation. Nuclear γH2AX foci were stained with fluorophore-labelled antibody and DAPI as nuclear counterstain. Cells were investigated by immunofluorescence microscopy (> 50 cells imaged per condition) and the number of γH2AX foci per nucleus counted with error bars indicating the standard deviation. Unfortunately, we could not obtained biological replicates