Fig. 4
Therapeutic efficacy, local control, and overall survival. a GBM tumor growth in control animals and animals irradiated according to the treatment plans shown in Fig. 2e as determined by Lx Hx W volumetric method depicted in Fig. 3a. b Time-to-CT-progression analysis derived from tumor volumes as depicted in (a). Tumor volumes determined on d17 served as reference. p-values were derived from log rank tests and were subjected to Bonferroni-Holm multiple testing correction. c Kaplan-Meier survival analysis of animals in the described treatment groups. p-values were derived from log rank tests and were subjected to Bonferroni-Holm correction. d Pearson correlation analysis of CT progression and overall survival as shown in (b) and (c). e Exemplary contrast-enhanced CBCT scans of a control mouse (upper panel) and a mouse treated with 10 × 2 Gy (5× 5 mm2 collimation, lower panel) when moribund. 40 μm H&E stained tissue sections (left) of brains in intersecting planes similar to the ones shown in the CBCT scans. Arrowheads indicate the tumor. 3D volume renderings (right) display similar tumor size (red), shape, and localization within the brain (blue). f Tumor volumes of moribund control and radiotherapy-treated animals. p-value as calculated by exact Wilcoxon Rank test