Fig. 5

Validation of PAF1 occupancy at the IER5 promoter and enhancers in different situations. a Genome browser track examples showing ChIP-seq data of PAF1, H3K27ac, Pol II, and H3K4me1 at the loci of IER5 and the putative enhancer region (red dotted box) aligned to the human genome (GRCh37.p13) using Integrative Genomic Viewer (IGV 2.4.10). The yellow arrow refers to the promoter region (− 199 ~ + 1); the pink arrow refers to the first enhancer region (+ 16,561 ~ + 16,362); the green arrow refers to the second enhancer region (+ 23,662 ~ + 23,861); corresponding primers were designed according to these regions. “+” indicates downstream of the IER5 start codon. P, promoter; E1, enhancer 1; E2, enhancer 2. b and c ChIP-qPCR results of the occupancy of PAF1 at the two enhancers and the promoter of IER5 in the Siha and Hela cells in different situations. b PAF1 occupancy at the promoter and two enhancer regions in the IER5 gene with or without PAF1 depletion in Siha and Hela cells. c PAF1 occupancy at the promoter and two enhancer regions of the IER5 gene before or after Siha and Hela cell IR, N = 3 in each group. All data are expressed as the mean (S.D.): * P < 0.05; ** P < 0.01; and *** P < 0.001