Mechanisms of different response to ionizing irradiation in isogenic head and neck cancer cell lines

Radiation Oncology

Table 3 Radiation-induced gene expression of genes involved in DNA damage signaling pathway in isogenic parental FaDu, radioresistant FaDu-RR and radiosensitive 2A3 cells 5 h after 5 Gy irradiation compared to non-irradiated parental FaDu, radioresistant FaDu-RR and radiosensitive 2A3 cells, respectivelly

Gene^a	FaDu^b	P value^c	FaDu-RR^b	P value^c	2A3^b	P value^c	DNA damage signaling pathways
ATR			1.71	0.01			ATM/ATR signaling, DSB repair, apoptosis, cell cycle
BBC3			−2.00	0.05			apoptosis
BLM	2.26	0.04	2.69	0.005			DSB repair
CRY1			−1.54	0.02			other DNA repair genes
ERCC2			1.54	0.03			NER
GADD45A	2.02	0.01	2.36	0.004			other DNA repair genes
H2AFX			1.63	0.03			ATM/ATR signaling, DSB repair
MCPH1			1.66	0.003			cell cycle
MDC1	1.86	0.05	1.59	0.04			DSB repair, cell cycle
MSH3			1.60	0.04			mismatch repair
NBN			1.61	0.009			DSB repair
PMS1	2.20	0.02	2.15	0.02			mismatch repair
PRKDC	2.41	0.04					DSB repair, apoptosis
XPA			1.99	0.02			NER
XRCC2			2.10	0.05			DSB repair
XRCC3					−2.40	0.007	other DNA repair genes

^a Gene symbol according to HUGO Gene Nomenclature Committee; ^b Fold regulation in gene expression in 5 Gy-irradiated cells in comparison to non-irradiated cells. Positive fold regulation values indicate gene over-expression, negative fold regulation values indicate gene under-expression; ^c The P values were calculated based on a Student’s t-test of the replicate 2^{(− ΔΔ CT)} values for each gene in the 5 Gy-irradiated and non-irradiated cells, and differences in gene expression were considered significant for P values less than 0.05. DSB double-strand break, NER nucleotide excision repair

ISSN: 1748-717X