Fig. 2
From: Development of a high-throughput γ-H2AX assay based on imaging flow cytometry
Gating strategy for assessing γ-H2AX levels in the IDEAS® software. a Using the Gradient RMS feature in the brightfield (BF) channel, which indicate sharpness of an image, cells with optimal focus were selected. b Using the area and aspect ratio features in the brightfield channel, single cells were selected and doublet events were removed. c DNA positive cells were selected based on DRAQ5 positivity and DNA negative cells were removed. d Lymphocytes were selected based on their size using the BF area and SSC intensity features