Table 1 Primary antibodies used for western blotting (WB), immunocytochemistry (ICC) and immunohistochemistry (IHC)
From: Development and characterisation of acquired radioresistant breast cancer cell lines
Primary antibody target antigen | Antibody Details | Dilutions and applications | Antigen retrieval |
|---|---|---|---|
Anti-α tubulin | Mouse MAb; Abcam; ab7291 | 1:10,000 (WB) | N/A |
Anti-ERα | Mouse MAb; Dako; M7047 | 1:50 (WB, ICC, IHC) | Sodium citrate |
Anti-EGFR | Rabbit MAb; Cell Signalling Technology; 4267 | 1:1000 (WB); 1:50 (ICC, IHC) | EDTA |
Anti-HER2 | Rabbit MAb; Cell signalling Technology; 2242 | 1:1000 (WB); 1:50 (ICC, IHC) | Sodium citrate |
Anti-PgR (A/B) | Rabbit MAb; Cell Signalling Technology; 8757 | 1:1000 (WB) | N/A |
Anti-PgR | Mouse Mab; DAKO; M3569 | 1:150 (ICC, IHC) | EDTA |
Anti-AKT | Mouse MAb; Cell Signalling technology; 2920 | 1:1000 (WB) | N/A |
Anti-Phospho AKT | Rabbit PAb; Cell Signalling technology; 9271 | 1:1000 (WB) | N/A |
Anti-ERK | Rabbit PAb; Cell Signalling Technology; 9102 | 1:1000 (WB) | N/A |
Anti-Phospho ERK | Mouse MAb; Cell Signalling Technology; 9106 | 1:1000 (WB) | N/A |
Anti-ki67 | Mouse MAb; DAKO; M7240 | 1:150 (ICC, IHC) | Sodium citrate |
Anti-E-cadherin | Mouse MAb; BD Transduction; 610,182 | 1:50 (ICC, IHC) | Sodium citrate |
Anti-N-cadherin | Mouse MAb; BD Transduction; 610,921 | 1:150 (ICC, IHC) | Sodium citrate |
Anti-vimentin | Mouse Mab; Abcam; 8069 | 1:50 (ICC, IHC) | Sodium citrate |
Anti-SNAIL | Rabbit PAb; Abcam; 128,530 | 1:250 (ICC, IHC) | Sodium citrate |