Molecular signature of response to preoperative radiotherapy in locally advanced breast cancer

Radiation Oncology

Table 2 Gene expression analysis by qRT-PCR in an independent subset of 42 LABC tumor samples

#	Gene Symbol	Amplicon Length	Responders (R)(n = 30)		Non-responders (NR)(n = 12)		p-value	Fold change
#	Gene Symbol	Amplicon Length	mean	sd	mean	sd	p-value	Fold change
1	APOA1	63	5.99	1.96	7.55	1.37	0.0161**	0.34
2	CHEK2	109	−4.63	1.69	−4.18	1.06	0.390	0.73
3	IDO1	106	1.06	2.87	0.63	2.82	0.662	1.35
4	MAP3K4	89	−3.00	1.99	−1.78	1.28	0.0582*	0.43
5	MCM6	109	−5.12	1.98	−4.88	2.34	0.741	0.85
6	MMP14	92	7.85	2.85	9.61	2.03	0.0596*	0.30
7	ST3GAL4	60	−3.95	2.68	−3.38	3.32	0.566	0.67
8	WHSC1L1	67	−0.98	3.90	−1.16	4.61	0.979	1.13
9	XRCC2	66	−4.68	2.08	−4.04	2.13	0.455	0.64

Gene expression was determined by qRT-PCR in independent test set of 60 FFPE breast tumors, of wich 42 were retained for data anlysis. Represented data were interplate calibrated, normalized to B-Actin and log2 transformed. Normality was evaluated using Lilform test. p-values - level of significance according to the Student’s t-test or nonparametric Kolmogorov-Smirnov test (WHSC1L1 and ST3GAL4), FC fold change gene expression relative to ACTB between pRT responders (R) to nonresponders (NR) tumors measured by qPR-PCR; Fold change is shown on a linear scale
*p-value < 0.1; **p-value < 0.05

ISSN: 1748-717X