Fig. 2

TAE684 selectively augments radiotherapy-induced antiproliferative effects in ALK positive NSCLC. Cell proliferation after incubation with vehicle or TAE684 (40 nM) alone or in combination with irradiation (4 Gy) was evaluated in H3122 (a), LLC (b) and A549 (c) cells (left panel). Only in ALK-positive H3122 cells dual treatment with TAE684 and radiotherapy reduced cell proliferation by 56% as compared to radiotherapy alone (p < 0.01). Photomircographs of representative fields after 72 h (H3122 and A549) or 36 h (LLC) are shown on the right panel. Cell numbers were normalized to that of non-treated groups for each cell line. Bars indicate mean ± SD. * p < 0.05 and ** p < 0.01