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Fig. 2 | Radiation Oncology

Fig. 2

From: Carbon ion radiotherapy: impact of tumor differentiation on local control in experimental prostate carcinomas

Fig. 2

Comparison of histological sections for the H- (left column), HI- (middle column) and AT1-tumor (right column). Structural changes and differentiation level are detected by Hematoxylin / Eosin (H&E) staining (upper row). For visualization of hypoxic areas, pimonidazole was used (brown staining, 2nd row) and cell nuclei were counterstained with Hematoxylin. Vessel endothelium was stained with CD31 antibody in red (3rd row). The maturity of vessels was proven by a pericyte staining using a smooth muscle actin antibody. Magnification: 100× (1st and 2nd row) or 200× (3rd and 4th row). Scale bars are 100 μm

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