Fig. 1

Image-guided Microbeam Radiotherapy Method Abstract. a: Lateral radiograph of mouse head was taken to locate the bregma. The head was stabilized using two ear-bars and teeth wire. Embed steel bead served as the fiducial marker. b: The skull outlines were sketched over the same radiograph. The anatomical place of hippocampus is shown regarding the bregma. d: Schematic lateral view of mouse skull with a cut along the corpus callosum at midline. The gray line demonstrates the microbeam. In our device, the microbeam planes intersect with the vertical plane at a slight angle of 8 degrees. The center of the treatment was placed 2 mm posterior to the bregma (c) and 2.5 mm inferior to the top of the skull [50]. e: The side (top) and top view (bottom) of mouse under irradiation. The head was fixed by ear bars and tooth wire. Gafromic EBT-3 film was placed on top of the mouse head (entrance plan) to record the beams and generate the dose profile (f)