Skip to main content
Fig. 3 | Radiation Oncology

Fig. 3

From: PARP-1 depletion in combination with carbon ion exposure significantly reduces MMPs activity and overall increases TIMPs expression in cultured HeLa cells

Fig. 3

Expression of MMP-2 and MMP-9 protein after carbon ion exposure (0- 4 Gy) in H-vector and HsiI cells. a Typical western blot of MMP-2 and beta actin (used as internal control). b Band intensities of western blot of MMP-2 were measured by ImageJ software and normalized with respect to beta actin and plotted as % expression of MMP-2 considering MMP-2 expression in un-irradiated H-vector as 100 %. c Expression of MMP-2 was also measured by IF technique. Intensities of the immunostained cells for MMP-2 were measured by ImageJ software, normalized with respect to beta actin and plotted as mentioned above. Typical immunostained cells are shown in Additional file 2. d Expression of MMP-9 was measured by IF technique and typical immunostained cells are shown here. Scale bar represents 20 μm. e Intensities of the immunostained cells for MMP-9 were measured by ImageJ software, normalized with respect to beta actin and plotted as mentioned above. Each bar diagram represents the mean ± SD of three independent experiments

Back to article page

Radiation Oncology

ISSN: 1748-717X

Contact us