An expression analysis of markers of radiation-induced skin fibrosis and angiogenesis in wound healing disorders of the head and neck

Table 1 Primer sequences and GenBank® accession numbers^a of examined genes

Gene	Accession no.	Sequence 5´to 3		Efficiency in %^b
Human procollagen alpha 1 (α-PC)	NC_000077.6	Forward	CTCGAGGTGGACACCACCCT	104.437
		Reverse	CAGCTGGATGGCCACATCGG
Tumor growth factor beta 1 (TGF-β1)	NC_010448.3	Forward	TGGCGATACCTCAGCAACC	95.488
		Reverse	CTCGTGGATCCACTTCCAG
Human von Willebrand Factor (vWF)	NM_000552	Forward	GTGACGTGTAATGGGAGACT	95.374
		Reverse	GTCATTGGCTCCGTTCTCAT
GAPDH	NG_007073.2	Forward	GAGTCAACGGATTTGGTCGT	96.783
		Reverse	TTGATTTTGGAGGGATCTCG

^aGenBank® is the National Institutes of Health (NIH, USA) genetic sequence database, an annotated collection of all publicly available DNA sequences [38]
^bχ=-1+10^(-1/slope)

ISSN: 1748-717X