Fig. 2
From: The Focinator - a new open-source tool for high-throughput foci evaluation of DNA damage
The macro automates the setting of the threshold and the contains an automated ROI selection. Figure 2 shows the calculation process frozen at the point of completed ROI selection. The ROI selection is necessary for the measurement of ROI area, intensity information (mean, maximum and minimum) and the foci count of each ROI (e.g. nucleus). Adjusting the threshold is the first step of ROI selection. The ROIs are marked by signal intensity-triggered selection of the areas. This selection and ROI marking is based on ImageJ “Create Selection” algorithm with options including filters for edge ROI exclusion, minimum and maximum size, watershed for overlapping objects and consideration of circularity