Fig. 1

Alteration of SHP-1 expression in human nasopharyngeal carcinoma (NPC) cell lines CNE-1 and CNE-2 by lentivirus-mediated RNA interference and overexpression, respectively. a SHP-1 protein expression in CNE-1 and CNE-2 cells was determined by western blot. b CNE-1 and CNE-2 cell survival according to radiation dose determined by colony formation assay. *P < 0.05, **P < 0.01, ***P < 0.001 CNE-1 vs. CNE-2. CNE-1: non-transduced CNE-1 cells; CNE-1-scramble shRNA: CNE-1 cells transduced with lentivirus-mediated scramble shRNA; CNE-1 SHP-1 shRNA: CNE-1 cells transduced with lentivirus-mediated SHP-1 shRNA; CNE-2: without transduced CNE-2 cells; CNE-2-empty vector: CNE-2 cells transduced with lentivirus vector; CNE-2 SHP-1 overexpression: CNE-2 cells transduced with lentivirus-mediated SHP-1 overexpression. According to fluorescence microscopy, transduction efficiency in CNE-1 (c) and CNE-2 (d) cells was >90 % at 2 days after transduction (magnification: ×400). e SHP-1 mRNA expression were determined by real-time RT-PCR. Relative mRNA expression was normalized to CNE-1 or CNE-2, and β-actin was used as an inner control. SHP-1 protein expression in CNE-1 (f) and CNE-2 (g) cells was determined by western blot. β-actin was used as control. h Quantitative results of western blot are shown as mean ± standard deviation (SD) from three independent experiments. ^** P < 0.01, ^*** P < 0.001 vs. CNE-1 or CNE-2; ^## P < 0.01, ^### P < 0.001 vs. CNE-1-scramble shRNA or CNE-2-empty vector