Figure 3

Strain dependent bronchoalveolar lavage cytokine levels in acute radiation response. Bronchoalveolar lavage fluid was obtained from control and irradiated mice at 6 hours, 1 day and 7 days post-irradiation. A) Bronchoalveolar lavage cytokine concentrations measured using the Multiplex assay. Results are presented as mean ± SE for groups of n = 4-5 mice. Significant variation in inbred strain measures by ANOVA indicated. B) Pearson correlation coefficients of late lung disease phenotypes with bronchoalveolar lavage cytokine levels (correlation coefficients between the fibrosis score as percent pulmonary fibrosis and the cytokine concentrations were R = −0.77, −0.80 and p-values 0.07, 0.05 respectively) C) Linear regression of the pulmonary fibrosis score against the lavage levels of Il17 and Ifnγ recorded at the day 7 time point. A significant interaction (p = 0.008) between the Il17 and Ifnγ measures is indicated by the intersecting regression lines of the fibrosis score against level of each cytokine.