Figure 2

WTI triggers distinct time-dependent changes in the T cell compartment of peripheral lymphoid organs. C57BL/6 mice received 0 Gy or 15 Gy whole thorax irradiation. At different time points, cells were isolated from cervical lymph nodes (cLN) and spleen (TSC) and stained with antibodies against (A/B) CD3+ T cells (cLN and spleen), (C/D) CD4+ T-helper cells (cLN and spleen), (E/F) CD8+ T cells (cLN and spleen) as indicated. Cells were analyzed by flow cytometry and timelines of the indicated cell populations are shown with mean values ± SEM of percentages calculated on lymph node cells (LNC) or total spleen cells (TSC). Cells of 6-9 mice per group were analyzed, * p ≤ 0.05; ** p ≤ 0.01; *** p ≤ 0.001, two-way ANOVA followed by post-hoc Bonferroni test.