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Figure 2 | Radiation Oncology

Figure 2

From: A non-linear detection of phospho-histone H2AX in EA.hy926 endothelial cells following low-dose X-irradiation is modulated by reactive oxygen species

Figure 2

ROS levels in EA.hy926 ECs following low-dose X-irradiation. (A) Representative flow cytometer dot plots forward scatter (FSC) vs side scatter (SSC) for each treatment regimen. R-1 indicates the population of cells used for the analysis of 2′,7′-dichlorodihydrofluoresceindiacetate (DCF) fluorescence. (B) Representative histograms showing an increase of DCF fluorescence as a marker of ROS production in TNF-α stimulated, non-irradiated (blue line) and irradiated (0.5 Gy, red line) cells. Non-DCF treated cells (black line) served as a control and were subtracted in every quantification. (C) Quantification of relative DCF-fluorescence in EA.hy926 ECs at 24 h after irradiation with the doses indicated. Data represent means ± SD (n = 4). *p < 0.05 vs. 0.3 Gy irradiated EC.

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Radiation Oncology

ISSN: 1748-717X

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