Figure 5

Prediction and confirmation of the target genes predicted for both let-7i and miR-21in rat lung type II pneumocyte cells. The cells were co-transfected with a negative control, let-7i and miR-21 mimics or inhibitors, respectively; (A/B) The relative mRNA expression level of the target genes (TGFBR1/TGF-β pathway) of let-7i mimics and inhibitors in cultured rat lung type II pneumocyte cells 48 h after transfection. (C/D) The relative mRNA expression level of the target genes (SMAD7/ TGF-β pathway) of miR-21 mimics and inhibitors in cultured rat lung type II pneumocyte cells 48 h after transfection. (E) Western blot analysis of TGFBR1/SMAD7 protein level in rat lung type II pneumocyte cells transfected as described in A for 72 hrs. Quantification of the bands’ intensity was shown on the right. All experiments were repeated at least three times independently. The results are presented as means ± S.E.M. *P < 0.05.