Figure 5

Silencing of eIF4B did not change radiation-induced reduction of Mcl-1 and apoptosis induction. Jurkat cells were transfected with 1μM eif4b siRNA by electroporation. 2 days later, cells were irradiated with 10 Gy. (A) Lysates were made 24 h after IR. eIF4B and Mcl-1 levels were analyzed by densitometry and normalized to the respective levels of non-irradiated cells transfected with non-targeting siRNA. Dissipation of mitochondrial membrane potential (ΔΨm low, B) and DNA fragmentation (sub G1, C) were analyzed by flow cytometry 24 h and 48 h after IR, respectively. Silencing of eIF4B had no impact on IR-induced Mcl-1 decline and apoptosis. Flow cytometric data shows mean values ± S.D. (n = 3), n.s. indicates no significance (p > 0.05).