Figure 2

Irradiation system. (a) Multiwell microplate containing the MCTS ready for in vitro testing. The spheroids were seeded into low-attachment multiwell plates containing fresh medium alone or supplemented with 20 μM of cisplatin. The medium was removed and substituted with 250 μl of fresh culture medium after a 72-h continuous exposure and the plates were once again placed in a CO₂ incubator at 37°C for 20 days during which time medium was changed daily. (b, c) Plexiglass phantom containing a microtiter plate was positioned on the treatment couch of the Elekta Synergy platform, the same machine used in daily clinical practice for the radiotherapeutic treatment of patients and periodically subject to specific quality assurance checks.