Figure 5
From: The role of IL-6 in the radiation response of prostate cancer
IL-6 is linked to MDSC recruitment. Effects of IL-6 and irradiation on MDSC recruitment in tumors were evaluated by (a) FACS of spleen using GrI-CD11b staining, and (b) immunofluorescence of tumor (DAPI, blue; CD11b, red) in mice bearing tumors (tumor size 300 ~ 350 mm3) with or without IL-6–silencing vectors or 48 h after 15-Gy irradiation or sham-irradiation. Representative images are shown. Quantification of CD11b expression was calculated as the number of cells positive for CD11b immunofluorescence divided by the total number of cells for each condition. Y-axis represents the ratio normalized to the value of TRAMP-C1 cells. Columns are the means of three independent experiments; bars, SD. *, P < 0.05. The effect of IL-6 on MDSC recruitment was further evaluated by (c) FACS of the spleen and (d) immunofluorescence of tumor specimens in pre-irradiated mice bearing tumors (with or without IL-6 stimulation) for 2 weeks.