Engineering cell-fluorescent ion track hybrid detectors

Radiation Oncology

Table 1 Cell-coating experiments

Cell line	Seeding density [1/well]	Fibronectin [ng/ml]	Cell-coating
			> 24 h	> 48 h	> 120 h
SMA-560	10 000	2.5, 5, 10, 20	Single cells, no network	Single cells, no network (*)	Network of overlapping cells
	150 000	2.5, 5, 10, 20	network of overlapping cells
	150 000	-	-	-	-
U87	10 000	2.5, 5, 10, 20	single cells, no network	single cells, no network (*)	single cells, no network
	150 000	2.5, 5, 10, 20	loosely packed network
	150 000	-	loosely packed network
PC3	200 000	2.5	-	-	-
	200 000	-	tightly packed monolayer
A431	200 000	2.5	tightly packed monolayer
	200 000	-	tightly packed monolayer
A549	50 000	-	-	tightly packed monolayer
	150 000	1, 2.5, 5	tightly packed monolayer
	150 000	-	tightly packed monolayer

The table shows the results of cell coating on the surface of FNTD crystals using different cell lines with and without a fibronectin intermediate layer. Each well contained 500 μ l. Cell culture medium was exchanged every 48 h. (*) Proliferation rate is lower compared to cells grown on the untreated bottom of a culture well.

ISSN: 1748-717X