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Table 1 Cell-coating experiments

From: Engineering cell-fluorescent ion track hybrid detectors

Cell line Seeding density [1/well] Fibronectin [ng/ml] Cell-coating
    > 24 h > 48 h > 120 h
SMA-560 10 000 2.5, 5, 10, 20 Single cells, no network Single cells, no network (*) Network of overlapping cells
  150 000 2.5, 5, 10, 20 network of overlapping cells   
  150 000 - - - -
U87 10 000 2.5, 5, 10, 20 single cells, no network single cells, no network (*) single cells, no network
  150 000 2.5, 5, 10, 20 loosely packed network   
  150 000 - loosely packed network   
PC3 200 000 2.5 - - -
  200 000 - tightly packed monolayer   
A431 200 000 2.5 tightly packed monolayer   
  200 000 - tightly packed monolayer   
A549 50 000 - - tightly packed monolayer  
  150 000 1, 2.5, 5 tightly packed monolayer   
  150 000 - tightly packed monolayer   
  1. The table shows the results of cell coating on the surface of FNTD crystals using different cell lines with and without a fibronectin intermediate layer. Each well contained 500 μ l. Cell culture medium was exchanged every 48 h. (*) Proliferation rate is lower compared to cells grown on the untreated bottom of a culture well.