Figure 1

Elevated Artemis levels confer a radioprotective effect against High and Low LET radiation. Western blots of lysates from human HEK293 cells carrying integrated vectors harboring tetracycline-inducible Artemis expression constructs (293Art), or control vector (HEK293), probed with antibodies recognizing Artemis (top) or tubulin for loading controls (bottom) (1A-inset). Results from a clonogenic survival experiment using HEK293 (open circles) and 293Art Artemis over expressing cells (solid triangles), stained and colonies scored 14 days after exposure to varied doses of X-rays (A) compared to a BrdU proliferation experiment set up in parallel and harvested 48 h post exposure (B). Results from BrdU proliferation experiments following high LET exposures in the form of iron nuclei (C) or titanium ions (D) carried out using the same dose range as X-ray experiments and processed at the same time points.