Identification of stable endogenous control genes for transcriptional profiling of photon, proton and carbon-ion irradiated cells

Table 2 Control genes ranked in order of their expression stability*

A431	M	A549	M	BxPC3	M
PGK1-RPL37A	0.02	RPLPO-UBC	0.12	RPL37A-RPLPO	0.03
PSMC4	0.04	GAPDH	0.13	CASC3	0.03
GUSB	0.07	MT-ATP6	0.16	RPL30	0.08
RPLPO	0.08	CASC3	0.19	UBC	0.08
UBC	0.09	PES1	0.27	RPS17	0.08
TBP	0.12	RPS17	0.31	EIF2B1	0.10
GAPDH	0.17	ACTB	0.32	ACTB	0.12
PPIA	0.16	TBP	0.42	POP4	0.13
ABL	0.16	PPIA	0.39	PSMC4	0.13
HPRT1	0.17	HMBS	0.41	PPIA	0.13
RPL30	0.17	PSMC4	0.46	ABL	0.14
PUM1	0.19	ABL	0.51	PGK1	0.14
ELF1	0.20	POLR2A	0.54	TBP	0.14
POLR2A	0.22	PUM1	0.60	GUSB	0.15
CDKN1A	0.23	RPL30	0.64	MRPL19	0.17
POP4	0.28	EIF2B1	0.65	CDKN1A	0.17
PES1	0.32	HPRT1	0.66	HPRT1	0.18
MT-ATP6	0.33	CDKN1B	0.67	PUM1	0.20
CASC3	0.32	MRPL19	0.65	HMBS	0.21
RPS17	0.33	RPL37A	0.68	MT-ATP6	0.21
HMBS	0.34	ELF1	0.73	B2M	0.23
EIF2B1	0.37	GUSB	0.87	GAPDH	0.25
YWHAZ	0.39	YWHAZ	0.88	PES1	0.27
CDKN1B	0.40	POP4	0.92	POLR2A	0.27
MRPL19	0.43	PGK1	0.97	YWHAZ	0.32
ACTB	0.48	GADD45A	1.04	ELF1	0.33
GADD45A	0.51	B2M	1.01	TFRC	0.36
IPO8	0.52	TFRC	1.22	IPO8	0.37
B2M	0.52	CDKN1A	1.28	18S	0.41
TFRC	0.64	18S	1.27	GADD45A	0.45
18S	0.96	IPO8	1.34	CDKN1B	0.58

*M values increasing from top to bottom; the two most stable control genes in each cell type, for example RPLPO and UBC in A549, cannot be ranked in order because of the required use of gene ratios for gene-stability measurements.

ISSN: 1748-717X