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Table 2 ANOVA of mean variance

From: Factors influencing heterogeneity of radiation-induced DNA-damage measured by the alkaline comet assay

ANOVA of mean variances
Sample MVar Sample MVar F-value p-value
0Gy (I) 47.63 0Gy (II) 83.81 2.493 0.153
0Gy (I) 47.63 0Gy (III) 23.03 2.551 0.149
0Gy (II) 83.81 0Gy (III) 23.03 11.624 0.009
4Gy (I) 86.53 4Gy (II) 284.02 23.365 0.001
4Gy (I) 86.53 4Gy (III) 106.01 1.807 0.216
4Gy (II) 284.02 4Gy (III) 106.01 9.506 0.002
0Gy (I) 47.63 4Gy (I) 86.53 4.328 0.071
0Gy (II) 83.81 4Gy (II) 284.02 21.728 0.002
0Gy (III) 23.03 4Gy (III) 106.01 70.647 <0.001
4Gy (I) 86.53 4Gy+15min (I) 48.96 10.140 0.013
4Gy (I) 86.53 4Gy+45min (I) 31.92 20.575 0.002
4Gy+15min (I) 48.96 4Gy+45min (I) 31.92 9.887 0.014
2x4Gy (IV) 120.51 2x4Gy+15min (IV) 138.36 0.947 0.359
2x4Gy (IV) 120.51 2x4Gy+45min (IV) 67.67 31.585 <0.001
2x4Gy+15min (IV) 138.36 2x4Gy+45min (IV) 67.67 14.608 0.005
2x4Gy (IV) 120.51 4Gy (I) 86.53 6.839 0.031
2x4Gy+15min (IV) 138.36 4Gy+15min (I) 48.96 26.039 0.001
2x4Gy+45min(IV) 67.67 4Gy+45min (I) 31.92 19.795 0.002
  1. Analysis of variance (ANOVA) was performed for the following pairs (1.-6.) of fraction I-IV*. (1.) Influence of DNA structure, antioxidants on heterogeneity of DNA damage without irradiation (line 1–3) and (2.) with irradiation (line 4–6). (3.) Influence of different radiation doses (line 7–9), (4.) heterogeneity of samples with increasing repair periods after single (line 10–12) and double fractionated irradiation (line 13–15) and (6.) comparison of single vs. double fractionated irradiation after same repair periods (line 16–18). In brief, heterogeneity was always significantly increased in histone- and antioxidant-depleted cells (fraction II) and after single or double fractionated irradiation but decreased with repair time or addition of antioxidants. Related mean variance (five donors) and mean DNA damage were shown in Figure 3a
  2. Abbreviations: F = F-value, p = probability value. MVar = mean variance.
  3. * 200 leukocytes/sample of each donor were measured for calculation of DNA damage in %Tail DNA and variance. Mean variance was calculated from 5 donors.