Figure 5

Secretion of matrix metalloproteases and endogenous inhibitors by CAFs. In panel (A), average determinations of the MMPs levels found in culture supernatants 4 to 6 days post-irradiation from 5 donors is represented (donors #1, #5, #7, #11, #14 in Table 1). Only MMPs that showed values above the detection limit of the assay are shown. Pale grey bars non-irradiated cultures; dark grey bars cells receiving (1 × 18 Gy). Statistically significant differences between control and irradiated cultures were observed for MMP-3 (p < 0.05), and in four out of five donors for MMP-1 (p = 0.06 all donors; p < 0.05 excluding donor-1). In panel (B), expression patterns of TIMP-1, -2, -3 from the same supernatants used in (A) are shown.