Figure 4

Induction of MIP-1α expression in RAW 264.7 cells. A. RAW cells were exposed either to 60 ng/mL LPS for 48 h, 40 ng/mL IL-4 for 12 h, or 60 ng/mL IL-13 for 8 h, followed by culture supernatant collection. Supernatant MIP-1α was assayed by ELISA. B. RNA was extracted from RAW cells treated as shown in A. MIP-1α mRNA levels were quantified using real-time RT-PCR, with an 8h control group. β-actin was used as an internal control. Calculation of fold values is described in Materials and Methods. Values are averages ± SD of two independent experiments each done in triplicates; (**) indicates p < 0.01 (one way ANOVA).