Figure 3

Recombinant MIP-1α as a potent chemoattractant for MΦ in vitro. Cells were exposed to 60 ng/mL LPS for 48 h, 40 ng/mL IL-4 for 12 h, or 60 ng/mL IL-13 for 8 h, followed by cell collection. MΦ chemotaxis was measured in a Transwell chamber with rMIP-1α at several concentrations. Results are expressed as cell number/horizon under a light microscope (250 times) Values are averages ± SD done in triplicates; Significant difference (p < 0.01) of chemotactic ability was obvious for different activated states of MΦ (one way ANOVA).