Figure 3

Kinetics of expression of γIR-induced DNA damage response proteins. SLGCs and directly derived FBS cultures were irradiated with the doses indicated. A. Expression levels of phosphorylated histone H2AX (γH2AX) detected by Western blot (representative result, upper panel) and quantification of γH2AX-signals using Image Quant TL (lower panels). B. Expression levels of p53 and its target gene p21 (WAF1/Cip1), as well as of phosphorylated p53 in cell lysates collected at the indicated times after irradiation. Actin levels are shown as control. In A and B, blots shown are representative of three independent experiments.