Figure 6
The effects of BO-1051 on radiation-induced tumor growth delay and prolongation of TTF (time to treatment failure) in nude mice bearing GBM-3 xenografts. When tumors reached 150 mm3, the nude mice with established GBM-3 flank xenografts were randomized into 4 groups: control (black circle), radiation (white circle), BO-1051 (black triangle) or BO-1051 plus radiation (white triangle). BO-1051 (50 mg/kg) was delivered via i.p. injection on days 0, 3 and 6, where day 0 begins on the day of randomization. Radiation (4 Gy) was delivered 24 h after the first injection of BO-1051 (day 1 after randomization), which corresponded to the same tumor size. Each group contained at least 8 mice. (A) Tumor growth rates for each treatment group were plotted as the mean relative tumor volume ± SD. Arrows indicate the time of BO-1051 and irradiation treatment. (B) Kaplan-Meier survival rates of nude mice with GBM-3 flank xenografts for each of the four treatments is depicted. Survival analysis was monitored daily. Treatment failure was defined as tumor size greater than 1500 mm3 or the development of severe necrosis requiring euthanasia.