Figure 4
Quantitative RT-PCR analysis of the expression of hypoxia regulated genes. Expression of carbonic anhydrase IX (CA9) (A) and lysyl oxidase (LOX) (B) in head and neck squamous cell carcinoma cells HNO97 under normoxia and hypoxia (2% O2) using the enzymatic GOX/CAT system and a hypoxia chamber. mRNA levels were measured by quantitative real time PCR. Columns, average from three independent measurements and show relative expression levels compared with cells at time point t = 0; Bars, SD. * p < 0.05.