Figure 4

Resveratrol (RSV) inhibits basal and ionizing radiation (IR)-induced Akt phosphorylation. A. Effects of IR alone: PC3 cells were treated with 0 or 8 Gy IR and lysed according to the indicated time points following IR exposure. Then cells were subjected to immunoblot analysis. B. RSV inhibits basal Akt phosphorylation: PC3 and 22RV-1 cells were treated with RSV (0, 2.5 or 5 μM) for 1 hour and subjected to lysis and immunoblot analysis using the indicated antibodies. C. RSV inhibits IR-induced Akt phosphorylation. PC3 and 22RV-1 cells were pre-treated with the indicated concentrations of RSV 1 hour prior to IR treatment. Cells were lysed 1 hour after IR exposure and subjected to immunoblot analysis. Shown are representative immunoblots of 4 independent experiments. D. Densitometric quantitation of Akt (S473) phosphorylation and total Akt was performed. P-Akt was normalized to T-Akt levels and presented as fold change compared to untreated control cells (mean ± SE) from 3-4 independent immunoblotting experiments as in C). * p < 0.05 compared to cells treated with IR alone.