Skip to main content
Figure 3 | Radiation Oncology

Figure 3

From: Radiosensitization and growth inhibition of cancer cells mediated by an scFv antibody gene against DNA-PKcs in vitro and in vivo

Figure 3

Effect of anti-DPK3-scFv on cellular survival. (A) Immunohybridization analysis of anti-DPK3-scFv expression in HeLa cell clones (C1 - C5) stably transfected with His-anti-DPK3-scFv-2 using an anti-His antibody. (B) Growth rates for HeLa, HeLa-pcDNA and DPK3-scFv-2-transfected HeLa cells (HeLa-DPK3-scFv) under normal growing condition. (C) Clonogenic assays of cells survivals for HeLa, HeLa-pcDNA and the clones (scFv-C3, C4, C5) of DPK3-scFv-2-transfected HeLa cells after 4 Gy γ-ray irradiation. * P < 0.05, #P < 0.01 as compared with control HeLa-pcDNA cells. (D) Cell survival curves of HeLa, HeLa-pcDNA and HeLa-DPK3-scFv cells (clone 2) post-irradiation. (E) Caspase-3 activity assays of HeLa, HeLa-pcDNA and HeLa-DPK3-scFv cells post-4 Gy irradiation. * P < 0.05 as compared with control HeLa or HeLa-pcDNA cells.

Back to article page

Radiation Oncology

ISSN: 1748-717X

Contact us