Figure 1
DCQ reduces the viability of HCT116 cells more so under hypoxia than normoxia. (A) The effect of hypoxia on HCT116 (p53+/+, p53-/-, p21-/-) cell viability after 6, 12, or 24 hrs of exposure to 1% O2. Cells were plated in 96 well plates at 1.2 × 105 cells/ml and treated at 50% confluency. Viability was determined using Cell Titer 96 non-radioactive proliferation assay. (B) Dose-dependent decrease in the viability of cells exposed to DCQ for 6 hrs and cultured under normoxia or hypoxia. Values are averages ± SD of two independent experiments each done in triplicates; (*) indicates p < 0.05 (one way ANOVA). ■Normoxia □ Hypoxia. The experiment was repeated three times each in quadruplicates.