Figure 8

Effects of DCQ and IR on the expression levels of p53, p-p53, p21 (A), Bax/Bcl2 (B), p-Akt and Cox-2 (C) proteins. DLD-1 cells were treated under oxic and hypoxic conditions with 5 μM DCQ, 2 Gy IR or combinations. After 24 hours, 40 μg cell lysates were subjected to SDS-PAGE. Fold induction of protein levels was based on densitometry measurments. Protein levels in treated cells were defined as percentage of control. All plots were re-probed with GAPDH to ensure equal protein loading.