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Figure 11 | Radiation Oncology

Figure 11

From: The membrane targeted apoptosis modulators erucylphosphocholine and erucylphosphohomocholine increase the radiation response of human glioblastoma cell lines in vitro

Figure 11

ErPC and ErPC3 decrease the number of clonogenic T98G cells and increase radiation-induced eradication of clonogenic T98G cells. Effects of ErPC and ErPC3 alone or in combination with ionizing radiation on clonogenic cell survival was determined by standard colony formation assays. 24 h after plating T98G cells were treated with increasing concentrations of (A) ErPC or (C) ErPC3 alone or (B, D) in combination with ionizing radiation with 0, 2.5, 5 and 10 Gy. In the latter case, ErPC or ErPC3 were added 10 min after irradiation. Subsequently, cells were incubated under standard culturing conditions up to 4 weeks. For determination of colony formation cells were fixed in 3.7% paraformaldehyde and 70% ethanol and stained with 0.05% Coomassie blue. Colonies composed of at least 50 cells were counted. Data represent means ± s.d., n = 3

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