Fig. 5

Colony forming assay for LS174T, LoVo, A549 and MDA-MB-231 cells. LS174T (A), LoVo (B), A549 (C) and MDA-MB-231 (D) cancer cells were treated with 0.1 mM diclofenac (Dic) for 48 h and then were irradiated (0–6 Gy). The cells were allowed to form colonies in drug-free medium. Two way ANOVA was used to evaluate significant differences (*p ≤ 0.05***, p ≤ 0.001). LS174T (E), LoVo (F), A549 (G) and MDA-MB-231 (H) were kept untreated or were treated with 0.1 mM diclofenac, 1 or 5 µM 5-fluorouracil (5-FU) or with diclofenac and 5-FU. After 48 h the medium was changed and cells were allowed to form colonies. The colony forming assay represents the results of at least 3 independent experiments. The one way ANOVA test was used to evaluate significant differences (**p ≤ 0.01, ***p ≤ 0.001)