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Fig. 4 | Radiation Oncology

Fig. 4

From: The radiation- and chemo-sensitizing capacity of diclofenac can be predicted by a decreased lactate metabolism and stress response

Fig. 4

Effects of diclofenac on membrane Hsp70 expression. Plasma membrane Hsp70 expression on untreated and diclofenac treated (0.1, 0.2 mM for 48 h) LS174T (A), LoVo (B), A549 (C) and MDA-MB-231 (D) cancer cells. Data present the proportion of positively stained cells of at least 3 independent experiments. (E) Representative gating strategy to determine membrane Hsp70 expression on viable tumor cells. Left, side scatter (SSC)/forward scatter (FSC) dot plot histogram to identify the tumor cell population based on size (FSC) and granularity (SSC); middle, gating of Propidium Iodide (PI) negative, viable tumor cells (96.0%); right, overlay of two histograms representing membrane Hsp70 positive tumor cells (43.1%) using cmHsp70.1-FITC monoclonal antibody (mAb, gray) and the negative control (white histogram) using an isotype-matched mAb (anti-mouse IgG1-FITC). The one way ANOVA test was used to evaluate significant differences (*p ≤ 0.05, **p ≤ 0.01)

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Radiation Oncology

ISSN: 1748-717X

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