Fig. 1

Diclofenac inhibits cell viability and c-MYC expression in LS174T, LoVo, A549 and MDA-MB-231 cancer cells. (A-D) Toxicity assay of LS174T (A), LoVo (B), A549 (C) and MDA-MB-231 (D) cancers cells treated with different diclofenac concentrations (0, 0.1, 0.2 and 0.4 mM) for 24 and 48 h. Two way ANOVA was used to evaluate significant differences (*p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001). (E-G) Representative immunoblot showing the expression of c-MYC 48 h after diclofenac treatment in LS174T (E), LoVo (F) and A549 (G) cancer cells. Quantification of the c-MYC signals of at least 3 independent experiments are shown in the bar charts above (*p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001)