Figure 3From: Reduced contribution of thermally labile sugar lesions to DNA double strand break formation after exposure to heavy ionsInduction of TLSL in naked DNA exposed to62Ni ions is suppressed by DMSO.Exponentially growing M059K cells were embedded in agarose and subjectedto LTL. Generated “naked” DNA was exposed to 5 Gy of1 GeV accelerated 62Ni ions in the presence of 0%(A) or 2% (B) DMSO. Subsequently, agarose blocks wereincubated in TEN buffer at different temperatures for the indicatedperiods of time and analyzed by PFGE. Data represent the calculatedaverage and standard deviation from 4 determinations (agarose blocks) inone experiment.Back to article page