Figure 2
Pimonidazole immunofluorescence and64Cu-ATSM autoradiography. Pimonidazole immunofluorescence (A and C) and 64Cu-ATSM photoluminescence autoradiography (B and D) of tumor sections from a fibrosarcoma (A and B) and an undifferentiated sarcoma (C and D). The fibrosarcoma displayed high and heterogeneous pimonidazole immunofluorescence staining (A) whereas lower and more homogeneous pimonidazole staining was found in the undifferentiated sarcoma (D). A strong correlation (rs = 0.72; p < 0.001) with 64Cu-ATSM was found in the fibrosarcoma (A vs. B) whereas this was not the case in the undifferentiated sarcoma (C vs. D)