Factors influencing heterogeneity of radiation-induced DNA-damage measured by the alkaline comet assay

Radiation Oncology

Table 3 Absolute DNA damage in %Tail DNA of fraction I-IV

n	Fraction	[Gy]	Repair [min]	%Tail DNA of five donors					Mean %
				Donor1	Donor2	Donor3	Donor4	Donor5	Tail DNA
1	I	0	0	5.91	5.56	4.60	7.45	5.89	5.88
2	II	0	0	20.19	24.39	16.66	12.38	17.31	18.19
3	III	0	0	6.56	6.68	6.19	6.82	7.83	6.82
4	II	4	0	64.44	62.09	57.45	61.83	60.54	61.27
5	III	4	0	23.02	31.20	34.51	37.96	34.83	32.30
6	I	4	0	24.90	28.56	25.95	25.91	27.17	26.50
7	I	4	15	11.45	12.22	12.83	11.11	10.99	11.72
8	I	4	45	7.75	7.89	6.61	6.13	8.89	7.45
9	IV	2x4	0	30.05	29.58	31.94	25.88	29.27	29.34
10	IV	2x4	15	19.11	19.16	21.39	19.92	20.95	20.11
11	IV	2x4	45	12.02	11.45	9.79	15.54	12.90	12.34

Mean DNA damage in %Tail DNA of donor leukocytes for fraction I-IV was represented^*. The highest levels of basal and initial DNA damages (at 0 and 4 Gy) were detected for nucleoids without antioxidants or histones (fraction II).
Abbreviations: n = number, Gy = Gray.
^* 200 leukocytes/sample of each donor were measured for calculation of DNA damage in %Tail DNA and variance. Mean variance was calculated from 5 donors.

ISSN: 1748-717X