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Figure 5 | Radiation Oncology

Figure 5

From: NVP-BEZ235 and NVP-BGT226, dual phosphatidylinositol 3-kinase/mammalian target of rapamycin inhibitors, enhance tumor and endothelial cell radiosensitivity

Figure 5

PI3K/mTOR inhibition enhances DNA damage in irradiated HUVEC. A, apoptosis assessment in HUVEC treated with BEZ235 and/or radiation. Previously starved HUVEC were treated with BEZ235 (50 nmol/L) for 1 h before irradiation. The medium was removed immediately following irradiation and was replaced by basal medium containing 1.5% FCS and a constant concentration of VEGF (10 ng/ml).. Annexin V-propidium iodide flow cytometry was used to identify cells in apoptosis or necrosis 72 h after irradiation. B, mean γH2AX foci number in HUVEC cells treated with BEZ235 (as above) and radiation at 24 h post-irradiation (n = 3). *, P < 0.05; **, P < 0.01; ***, P < 0.001 over DMSO-treated control.

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Radiation Oncology

ISSN: 1748-717X

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