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Figure 4 | Radiation Oncology

Figure 4

From: NVP-BEZ235 and NVP-BGT226, dual phosphatidylinositol 3-kinase/mammalian target of rapamycin inhibitors, enhance tumor and endothelial cell radiosensitivity

Figure 4

PI3K/mTOR inhibition affects downstream signaling and clonogenic survival in irradiated vascular endothelial cells. A, HUVEC were starved overnight and were treated with 50 nmol/L BEZ235 for 1 h before irradiation (4 Gy). Following irradiation, medium was replaced by either basal medium containing 1.5% FCS and 10 ng/ml VEGF (+VEGF) or growth factor-depleted medium (-VEGF). Cell lysates were collected 1 h post-irradiation and subjected to Western blot analysis. B, Clonogenic survival of HUVEC and HDMVC treated with BEZ235 (50 nmol/L) for 1 h before irradiation up to 1 h post-irradiation (n = 3). *, P < 0.05; **, P < 0.01 over DMSO-treated control.

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Radiation Oncology

ISSN: 1748-717X

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