Correlation between egfr expression and accelerated proliferation during radiotherapy of head and neck squamous cell carcinoma

Table 3 Numerical results of sub-site

Sub-site	Number of patients	Doubling time	Flow Cytometric (FCM)				Immunohistochemical (Hi)
Sub-site	Number of patients	Doubling time	days	D_p_(0.25)	D_p_(0.3)	D_p_(0.35)	days	D_p_(0.25)	D_p_(0.3)	D_{p (0.35)}
Oral Cavity	394 (18.2%)	T _D	6.6 (5.5-8.2)	0.35 (0.28-0.42)	0.29 (0.23-0.35)	0.25 (0.20-0.30)	5.9 (4.9-7.4)	0.39 (0.31-0.47)	0.33 (0.26-0.39)	0.28 (0.22-0.34)
Oral Cavity	394 (18.2%)	T _pot	3.9	0.59	0.49	0.42	2.3	1.00	0.84	0.72
Oropharynx	655 (30.3%)	T _D	6.6 (5.5-8.2)	0.35 (0.28-0.42)	0.29 (0.23-0.35)	0.25 (0.20-0.30)	5.9 (4.9-7.4)	0.39 (0.31-0.47)	0.33 (0.26-0.39)	0.28 (0.22-0.34)
Oropharynx	655 (30.3%)	T _pot	3.9	0.59	0.49	0.42	2.3	1.00	0.84	0.72
Hypopharynx	320 (14.8%)	T _D	6.1 (5.1-7.6)	0.38 (0.30-0.45)	0.31 (0.25-0.38)	0.27 (0.22-0.32)	4.6 (3.9-5.8)	0.50 (0.40-0.59)	0.42 (0.33-0.49)	0.36 (0.28-0.42)
Hypopharynx	320 (14.8%)	T _pot	3.6	0.64	0.53	0.46	1.8	1.28	1.07	0.92
Larynx	784 (36.3%)	T _D	12.9 (10.7-16.0)	0.18 (0.14-0.22)	0.15 (0.12-0.18)	0.13 (0.10-0.15)	14.3 (12.4-18.1)	0.16 (0.13-0.19)	0.14 (0.11-0.16)	0.12 (0.9-0.13)
Larynx	784 (36.3%)	T _pot	7.6	0.30	0.25	0.46	5.6	0.41	0.34	0.29

T_D (days) and D_prolif (Gy/day) for high EGFr expression group (<ⁱT_pot > =5.2 days by flow cytometry; <ⁱT_pot > =3.4 days by immunohistochemistry; T_K = 21 days). The ⁱT_D for each i sub-site (upper rows) is almost twice of ⁱT_pot by flow cytometry and more than double of ⁱT_pot by immunohistochemistry from literature (bottom rows) [15]. Consequently, a preclinical estimation of D_prolif by flow cytometry (left columns, bottom rows) or immunohistochemistry (right columns, bottom rows), results almost double and more than double if compared to the estimation of D_prolif obtained by mathematical model (left and right columns, upper rows), respectively. The 95 % confidence intervals are shown within the brackets.
Abbreviations: ⁱD_{p(0.25; 0.3; 0.35)} = D_prolif calculated with α = 0.25, α = 0.3, α = 0.35 Gy^-1 respectively, α/β = 10 Gy, d = 2 Gy.

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