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Figure 4 | Radiation Oncology

Figure 4

From: Radiosensitization and growth inhibition of cancer cells mediated by an scFv antibody gene against DNA-PKcs in vitro and in vivo

Figure 4

The effects of anti-DPK3-scFv on DNA-PKcs activity. (A) Immunohybridization analysis of total DNA-PKcs protein level in HeLa, HeLa-pcDNA and HeLa-DPK3-scFv cells. (B) In vitro detection of 4 Gy-induced DNA-PKcs activity using the Signa-TECT® DNA-Dependent Protein Kinase Assay System. Immunoblotting pattern (C) and relative p-Akt level (the ratio of p-Akt/Akt signals) (D) of Akt, p-Akt/S473 in HeLa, HeLa-pcDNA and HeLa-DPK3-scFv cells post-4 Gy irradiation. Immunoblotting pattern (E) and relative p-DNA-PKcs level (the ratio of p-DNA-PKcs/DNA-PKcs signals) (F) of DNA-PKcs, p-DNA-PKcs/S2056 in HeLa, HeLa-pcDNA and HeLa-DPK3-scFv cells post-8 Gy irradiation.

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Radiation Oncology

ISSN: 1748-717X

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