Figure 7
Induction of DNA damage in DLD-1 cells after treatment with DCQ, IR or combinations under oxic and hypoxic conditions. Cells were treated with 5 μM DCQ for 1 hour, 2 Gy IR or combinations. Immediately after treatment, DNA damage was assessed using alkaline single cell microgel electrophoresis (Comet) assay as mentioned in the "Materials and methods" section. A. The figure shows different grades of DNA fragmentation in DLD-1 cells. Magnification: 100×. B. An average of 100 cells per slide were counted and analyzed, and the mean of damaged cells is represented as the percentage of control untreated cells. C. Quantitative measurements of various comet assay end-points as analyzed using Comet Score software.